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Figure 5. RT-PCR confirmation of decreased MAGE-A expression by siRNA transfection in MDA-MB-231 cell line.

Journal: Neoplasma

Article Title: MAGE-A is frequently expressed in triple negative breast cancer and associated with epithelial-mesenchymal transition.

doi: 10.4149/neo_2016_006

Figure Lengend Snippet: Figure 5. RT-PCR confirmation of decreased MAGE-A expression by siRNA transfection in MDA-MB-231 cell line.

Article Snippet: MAGE-A siRNA (h) (SC-35843, SANTA CRUZ BIOTECHNOLOGy, USA), a pool of 6 different siRNA duplexes, was transiently transfected into MDA-MB-231 cells using FuGENE HD (E2311, Promega USA).

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Transfection